MNase-seq of S. cerevisiae derivative yGWG538: pZEVi-LYS2 induced (15... - SRA

SRX25740829: MNase-seq of S. cerevisiae derivative yGWG538: pZEVi-LYS2 induced (15nM Beta-Estradiol), Rep 2
1 ILLUMINA (Illumina NovaSeq 6000) run: 28.4M spots, 2.9G bases, 944.6Mb downloads

Design: Chromatin from formalin-crosslinked yeast was digested with MNase for 60 minutes to generate predominantly mono-nucleosome DNA fragments within the 100-200bp range. After de-crosslinking, DNA was purified with a phenol-based method and then further purified with a column (Clean & Concentrator, Zymo). Sequencing libraries were prepared from purified DNA samples using the automated KAPA general DNA-seq kit to introduce Illumina adapters and dual-indexing for multiplexed, paired-end (51 x 2) sequencing that reads the ends of each DNA fragment.

Submitted by: NYU Langone Health

Study: MNase-seq data from triplicate induction experiments with pZEVi-LYS2 yeast

PRJNA1149169 • SRP526970 • All experiments • All runs

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Paired-end sequencing of genomic DNA fragments purified from the MNase-digested chromatin of formalin-crosslinked yeast samples prepared in biological triplicate. Each biological replicate was treated with Beta-Estradiol (15nM or 1000nM) to induce pZEVi or left untreated (0nM) during growth at 30C in SC-Leu-Ura media that maintains Cas9/sgRNA episomes. The sgRNA was non-targeting in this strain background.

Sample:

SAMN43230667 • SRS22380527 • All experiments • All runs

Organism: Saccharomyces cerevisiae

Library:

Name: pZEVi-LYS2_15nM_Rep2

Instrument: Illumina NovaSeq 6000

Strategy: MNase-Seq

Source: GENOMIC

Selection: MNase

Layout: PAIRED

Runs: 1 run, 28.4M spots, 2.9G bases, 944.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR30280089	28,383,465	2.9G	944.6Mb	2024-10-11

ID:: 34773369

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